Size  100ug
Cat code  200390
Species Cross-reactivity  H
Key Application  WB,IP
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  62kDa
Sensitivity  This antibody detects endogenous levels TFIIH and does not cross-react with related proteins.
Immunogen  Purified recombinant human TFIIH protein fragments expressed in E.coli.
UniProt Number  P32780
GeneBank ID  NM_001142307
Subcellular location  Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername  Basic transcription factor 2 62 kDa subunit;Basic transcription factor 62 kDa subunit;BTF 2;BTF2;BTF2 p62;General transcription factor IIH polypeptide 1;General transcription factor IIH subunit 1;General transcription factor IIH,polypeptide 1,62kDa;gtf2h1;TF2H1;TF2H1_HUMAN;TFB1;TFIIH;TFIIH basal transcription factor complex p62 subunit.
Gene_symbol  P62;BTF2;TFB1;TFIIH.
Summary  
UniPort summary  Function  Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
Application Images :  
Western blot detection of TFIIH(N-terminus)in Hela,Hela NE & Jurkat cell lysates using TFIIH (N-terminus) antibody (1:300 diluted).Predicted band size: 62KDa.Observed band size: 62KDa.
Immunoprecipitation analysis of Hela cell lysates using GTF2H1 antibody.