Size  100ug
Cat code  200304
Species Cross-reactivity  H
Key Application  WB,IP
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG2b
Molecular Weight  70kDa
Sensitivity  This antibody detects endogenous levels of HSPA1A and does not cross-react with related proteins.
Immunogen  Purified recombinant human HSPA1A protein fragments expressed in E.coli.
UniProt Number  P08107
GeneBank ID  NM_005345.4
Subcellular location  Cytoplasm
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername  Heat shock 70 kDa protein 1/2
Short name=HSP70-1/HSP70-2
Short name=HSP70.1/HSP70.2
Gene_symbol  HSP72;HSPA1;HSP70I;HSPA1B;HSP70-1;HSP70-1A.
Summary  This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
UniPort summary  Function  In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
Application Images :  
Western blot detection of HSPA1A in Hela&HsHela cell lysates using HSPA1A antibody (1:1000 diluted).
Predicted band size: 70KDa
Observed band size: 70KDa
Immunoprecipitation analysis of Hela cell lysates using HSPA1A antibody.